According to state law, county officials will now appoint a fellow Republican who also resides in the district to take Hof's seat.
His campaign manager had predicted weeks ago that Hof would overcome his own death to clinch the vote because the district leans heavily Republican and even alluded that Hof would perform better due to the situation.
Hof had celebrated his birthday the day before with a party attended by porn star Ron Jeremy and other guests.
"There are a lot of Republicans who were uncomfortable voting for Dennis because of the nature of his business, and they now know that he is not the one who will be serving", he said.
The Love Ranch brothel that Hof owned became notorious as the place where former Laker Lamar Odom was found unconscious in 2015.
Hof, who starred in the HBO reality television show Cathouse about the prostitutes working at one of his establishments, was running as a Donald Trump-style Republican.
About 20 brothels operate in Nevada, mostly in rural areas.
He ran for office in 2016 as a Libertarian but lost. Most owners keep a lower profile than Hof did.
But much of Nevada's establishment GOP shunned him.
Steve Marcus/REUTERS/NewscomVoters in Nevada's Assembly District 36 must have really not liked the Democrat.
Hof, the self-described "Trump of Pahrump", was accused of rape and sexual assault by two of his former employees, according to the Review-Journal, citing a police report and interviews with the women.