The Army is not releasing the names of the deceased pending notification of the next of kin. No other casualties were reported.
"This is a day of sadness for Fort Campbell and the 101st Airborne", said 101st Airborne Division and Fort Campbell acting senior commander Brig.
'Our thoughts and prayers are with the Families during this hard time, ' he added.
The crash is the latest in a series of similar incidents involving US military aircraft that began on Tuesday.
An Army AH-64E Apache helicopter went down at about 9:50 pm on Friday, the 101st Airborne Division said.
Recent days have been particularly deadly for training across the military.
Finally, on April 4, a member of the Air Force Thunderbirds aerobatic team fatally crashed in an F-16 near Nellis Air Force Base, Nevada. The Army explained that the incident was under investigation. Aerospace expert Todd Harrison with the Center for Strategic and International Studies think tank in Washington, D.C., told Task & Purpose that pilots are often flying "old battle-worn aircraft".